live injection of activated PFA-treated CD4 T cells. injection of cells as indicated j snapshot of i.l. f–i Immunohistology of popliteal LNs directly after i.l. d, e Immuno-fluorescence microscopy (d) and quantitative analysis (e) of latex bead positioning in popliteal LN SCS after intra-lymphatic injection of fluorescent latex particles (6 µm, green 10 µm, blue 15 µm, pink) showing the farthest distance along the SCS between beads of the same size blue, counterstaining with anti-Lyve-1. b, c Quantitative analysis of SCS heights and transverse distances in resting (b) and in inflamed (c) pop LNs. White lines indicate measure points for SCS height and transverse distance between cells/fibers. The SCS represents a 3D sieve that acts as a mechanical barrier to arrest lymph-derived activated CD4 T cellsĪ In vivo imaging and three-dimensional reconstruction of an inflamed pop LN SCS after subcutaneous injection of TAMRA.
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